安捷倫1260熒光檢測器（G1321B） 介紹 The detector is designed for highest optical performance, GLP compliance and easy maintenance. It includes the following features: • flash lamp for highest intensity and lowest detection limit • multi-wavelength mode for on-line spectra • spectra acquisition and simultaneous multi-signal detection • optional cuvette is available and can be used for off-line measurements • easy front access to flow cell for fast replacement and • built-in wavelength accuracy verification. 參數 | Type | Specification | Comments | | Detection type | Multi-signal fluorescence detector withrapid on-line scanning capabilities and spectral data analysis | | | Performance specifications | Single wavelength operation: • RAMAN (H2O) > 500 (noise reference measured at signal) Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell • RAMAN (H2O) > 3000 (noise reference measured at dark value) Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell Dual wavelength operation: RAMAN (H2O) > 300 Ex 350 nm, Em 397 nm and Ex 350 nm, Em 450 nm, standard flow cell. |
| | Light source | Xenon Flash Lamp, normal mode 20 W, economy mode 5 W, lifetime 4000 h | | | Pulse frequency | 296 Hz for single signal mode 74 Hz for economy mode | | | Maximum data rate | 74 Hz | | | Excitation monochromator | Range: settable 200 nm - 1200 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator: concave holographic grating, F/1.6, blaze: 300 nm | | | Emission monochromator | Range: settable 200 nm - 1200 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator: concave holographic grating, F/1.6, blaze: 400 nm | | | Reference system | in-line excitation measurement | | | Timetable programing | up to 4 signal wavelengths, response time, PMT Gain, baseline behavior (append, free, zero), spectral parameters | | | Spectrum acquisition | Excitation or Emission spectra Scan speed: 28 ms per datapoint (e.g. 0.6 s/spectrum 200 – 400 nm, 10 nm step) Step size: 1 – 20 nm Spectra storage: All | | | Wavelength characteristic | Repeatability +/- 0.2 nm Accuracy +/- 3 nm setting | | | Flow cells | Standard: 8 ul volume and 20 bar (2MPa) pressure maximum, quartz Optional: Fluorescence cuvette for offline spectroscopic measurements with 1 mL syringe, 8 ul volume, quartz Bio-inert: 8 ul volume and 20 bar (2 MPa) pressure maximum, sapphire (pH 1—12) Micro: 4 ul volume and 20 bar (2 MPa) pressure maximum, quartz | | | Control and data evaluation | Agilent ChemStation for LC, Agilent Instant Pilot G4208A with limited spectral data analysis and printing of spectra | | | Analog outputs | Recorder/integrator: 100 mV or 1 V, output range > 100 LU, two outputs | 100 LU is the recommended range, see "FLD Scaling Range and Operating Conditions" | | Communications | Controller-area network (CAN), RS-232C, LAN, APG Remote: ready, start, stop and shut-down signals | | | Safety and maintenance | Extensive diagnostics, error detection and display (through Instant Pilot G4208A and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas. | | | GLP features | Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy, using the Raman band of water. | | | Housing | All materials recyclable | | | Environment | 0 – 40 °C constant temperature at <95 % humidity (non-condensing) | | | Dimensions | 140 mm x 345 mm x 435 mm (5.5 x 13.5 x 17 inches) (height x width x depth） | | | Weight | 11.5 kg (25.5 lbs) | | 配件與耗材 Overview of Maintenance Parts | p/n | Description | | G1321-60005 | Flow cell, 8 μL, 20 bar (pH 1–9.5) | | G1321-60015 | Flow cell, 4 μL, 20 bar (pH 1–9.5) requires a 0.12 mm i.d. capillary (e.g. p/n G1316-87318, 300 mm long), part of Capillary kit for 0.12 mm id (p/n G1316-68716) | | G5615-60005 | Bio-inert flow cell, 8 μL, 20 bar (pH 1–12) includes Capillary Kit Flow Cells BIO (p/n G5615-68755)) | | G5615-68755 | Capillary Kit Flow Cells BIO includes Peek Capillary i.d. 0.18 mm, 1.5 m lg and PEEK Fittings 10/PK (p/n 5063-6591) | | G1321-60007 | FLD Cuvette Kit, 8 μL, 20 bar | | 9301-0407 | Needle | | 9301-1446 | Syringe | | 5067-4691 | Front Panel DAD/VWD/FLD (1260/1290) | | 5041-8388 | Leak funnel | | 5041-8389 | Leak funnel | | 5041-8387 | Tube clip | | 5062-2463 | Corrugated tubing, PP, 6.5 mm id, 5 m | | 5062-2462 | PTFE Tubing flexible i.d. 0.8 mm, o.d. 1.6 mm, 2 m, re-order 5 m (flow cell to waste) | | 5181-1516 | CAN cable, Agilent module to module, 0.5 m | | 5181-1519 | CAN cable, Agilent module to module, 1 m | | G1369B or G1369-60002 | Interface board (LAN) | | 5023-0203 | Cross-over network cable, shielded, 3 m (for point to point connection) | | 5023-0202 | Twisted pair network cable, shielded, 7 m (for point to point connection) | | 01046-60105 | Agilent module to general purpose (Analog) | | G1351-68701 | Interface board (BCD) with external contacts and BCD outputs | Cuvette Kit | p/n | Description | | G1321-60007 | FLD Cuvette Kit, 8 μL, 20 bar includes | | 5062-2462 | PTFE Tubing flexible i.d. 0.8 mm, o.d. 1.6 mm, 2 m, re-order 5 m (flow cell to waste) | | PTFE Tubing flexible i.d. 0.8 mm, o.d. 1.6 mm, 2 m, re-order 5 m (flow cell to waste) | SST Fitting | | 0100-0043 | SST front ferrule | | 0100-0044 | SST back ferrule | | 0100-1516 | Fitting male PEEK, 2/pk | | 9301-0407 | Needle | | 9301-1446 | Syringe | Standard Accessory Kit
Accessory kit (p/n G1321-68755) contains some accessories and tools needed for the installation and repair/calibration of the detector. 
| Item | p/n | Description | | 1 | 5062-2462 | PTFE Tubing flexible i.d. 0.8 mm, o.d. 1.6 mm, 2 m, re-order 5 m (flow cell to waste) | | 2 | 0100-1516 | Fitting male PEEK, 2/pk | | 3 | G1315-87311 | Capillary column – detector 380 mm lg, 0.17 i.d., (includes SST ferrule front, SST ferrule back and SST fitting). | | 4 | 0100-0043 | SST front ferrule | | 5 | 0100-0044 | SST back ferrule | | 6 | 79814-22406 | SST Fitting | Capillary Kit Flow Cells BIO Capillary Kit Flow Cells BIO includes Peek Capillary i.d. 0.18 mm, 1.5 m lg and PEEK Fittings 10/PK (p/n 5063-6591) (p/n G5615-68755) includes
| p/n | Description | | 0890-1763 | Peek Capillary i.d. 0.18 mm, 1.5 m lg | | 5063-6591 | PEEK Fittings 10/PK | Overview of Main Assemblies 
| Item | p/n | Description | | 1 | G1321-65000 | Detector main board (FLM) - NEW (G1321B) | | 1 | G1321-69000 | EXCH Detector main board (FLM) (G1321B） | | 1 | G1321-66500 | Detector main board (FLM) - NEW (G1321A) | | 1 | G1321-69500 | EXCH Detector main board (FLM) (G1321A) | | | 1251-7788 | Hexagonal Nut for APG and COM port (4x) | | | 2940-0256 | Nut M14 — analog output | | | 2190-0699 | Washer — analog output | | | 5181-1516 | CAN cable, Agilent module to module, 0.5 m | | 2 | 0950-2528 | Power supply | | 3 | G1351-68701 | Interface board (BCD) with external contacts and BCD outputs | | 3 | G1369B or G1369-60002 | LAN Communication Interface board, | | 3 | G1369C or G1369-60012 | LAN Communication Interface board | | 2110-0004 | Fuse for BCD board, 250 mA | | 4 | 3160-1017 | Fan assembly | | 5 | G1321-69002 | Optical unit (exchange assembly) | | 6 | 2140-0600 | Flash lamp | | 7 | 1000-1000 | Mirror assembly | | 8 | G1321-60003 | EX Grating assembly (includes protection ring) | | 9 | G1321-60004 | EM Grating assembly (includes protection ring) | | 10 | 1970-0201 | Photo-multiplier Tube (PMT), | | 11 | 1000-1124 | Condenser assembly EM | | 12 | G1321-60005 | Standard flow cell, 8 μL, 20 bar | | 13 | 5061-3356 | Leak sensor | | 14 | G1321-61600 | Cable FLM - FLF board | Optical Unit Assembly
| Item | p/n | Description | | | G1321-60002 | Optical unit - NEW (comes with foam part and cable FLM/FLF) | | | G1321-69002 | Optical unit (exchange assembly) | | 1 | G1321-66512 | FLL Board (includes trigger pack) | | 2 | 2140-0600 | Flash lamp | | 3 | 1000-1136 | EX condenser Assembly | | 4 | G1321-08103 | Slit EX | | 5 | 1000-1000 | Mirror assembly | | 6 | G1321-60003 | EX Grating assembly (includes protection ring) | | 7 | G1321-60004 | EM Grating assembly (includes protection ring) | | 8 | G1321-60005 | Flow cell, 8 μL, 20 bar (pH 1–9.5) | | G1321-60015 | Flow cell, 4 μL, 20 bar (pH 1–9.5) | | G5615-60005 | Bio-inert flow cell, 8 μL, 20 bar (pH 1–12) includes Capillary Kit Flow Cells BIO (p/n G5615-68755)) | | G5615-68755 | Capillary Kit Flow Cells BIO includes Peek Capillary i.d. 0.18 mm, 1.5 m lg and PEEK Fittings 10/PK (p/n 5063-6591) | | G1321-60007 | FLD Cuvette Kit, 8 μL, 20 bar | | G1321-22400 | Screw for flow cell / cuvette | | 9 | G1321-08102 | Slit EM (also used for PMT) | | 10 | 1970-0201 | Photo-multiplier Tube (PMT), | | 11 | 1000-1124 | Condenser assembly EM | | 12 | G1321-66531 | Front-end Board (FLF) Rev D, underneath the optical (new) (requires firmware revision A.04.06 or above) | | 12 | G1321-69531 | FLF Board (requires firmware revision A.04.06 or above), FLR Reference Diode board G1321-66533 | | 13 | G1321-66533 | FLR Reference Diode board | | 14 | 1000-1121 | Diffuser plate (in front of the reference diode board FLR) | | 15 | 5062-8530 | Cutoff filter 295 nm | | 16 | 01046-42701 | Carbon filter mat | | 17 | G1321-61600 | Cable FLM - FLF board | | G1321-40103 | Foam Optics | | 18 | G1321-61602 | Cable chassis | | 19 | 0515-2574 | Screw M5 x 25 mm, for chassis cable | | | 0515-1052 | Screw 2.5 mm hex for monochromator, lamp pad, condenser #1, slits, etc | | | 0515-2548 | Screw M4 x 25 mm hex, for lamp housing | | | 0515-2549 | Screw M4 x 8 mm, for FLF board | Photo-muliplier Tubes and Cutoff Filters | p/n | Description | | 5061-3327 | Cutoff filter kit: 389 nm, 408 nm, 450 nm, 500 nm, 550 nm | | 5061-3328 | Cutoff filter kit: 380 nm, 399 nm, 418 nm, 470 nm, 520 nm | | 5061-3329 | Cutoff filter kit: 280 nm, 295 nm, 305 nm, 335 nm, 345 nm | | 1000-0822 | Cutoff filter 370 nm | | R928HA | Photo-multiplier Tube (PMT, 185 to 900 nm); contact Hamamatsu dealers | | R3788HA | Photo-multiplier Tube (PMT, 185 to 750 nm); contact Hamamatsu dealers | Lamp Cover Parts
| Item | p/n | Description | | 1 | G1321-08100 | Plate | | 2 | 0515-1052 | Screw 2.5 mm hex for monochromator, lamp pad, condenser #1, slits, etc. | | 3 | 1000-0999 | Quartz Window | Sheet Metal Kit
| Item | p/n | Description | | | G1321-68701 | Sheet metal kit includes case and top cover | | | 5022-2112 | Screw cover | Plastic Parts
| Item | p/n | Description | | 1 | 5067-4691 | Front Panel DAD/VWD/FLD (1260/1290) | | 2 | 5065-9985 | Cover kit (includes base, top, left and right) | | 3 | 5043-0207 | Name plate 1260 | Foam Parts
| Item | p/n | Description | | 1.2 | G1321-68702 | EPP foam kit, includes bottom and top rear | | 3 | G1321-68700 | Foam Pads (located underneath the optical), set of two | | 4 | 5041-8395 | Foam kit (includes top and bottom foam). | Power and Status Light Pipes
| Item | p/n | Description | | | 0950-2528 | Power supply | | | 0515-0910 | Screw M4 x 0.7, 8 mm lg, to fix power supply at rear panel | | | 2190-0409 | Washer | | 1 | 5041-8382 | Power switch light pipe | | 2 | 5041-8384 | Status light pipe | | 3 | 5041-8381 | Power switch button | | 4 | 5041-8383 | Power switch coupler | Leak Parts
| Item | p/n | Description | | 1 | 5061-3356 | Leak sensor | | 2 | G1321-40511 | Leak Pan | | 3 | 5041-8388 | Leak funnel | | 4 | 5041-8389 | Leak funnel holder | | 5.6 | 5042-9974 | Tubing Flex (1.5 m) | | 5.6 | 5062-2463 | Corrugated tubing, PP, 6.5 mm id, 5 m |
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